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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Teknoinfo Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) JK Unila (Jurnal Kedokteran Universitas Lampung)
Sutyarso Sutyarso
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

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Pengaruh Tepung Kedelai Kaya Isoflavon terhadap Testosteron Serum, Jumlah Sel Leydig dan Jumlah Sel Spermatogenik pada Tubuli Seminiferi Testis Tikus (Rattus norvegicus) Sussi Astuti; Sutyarso Sutyarso
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2643

Abstract

The objective of this experiment was to evaluate the effects of isoflavone-riched soybean flour with different levels of isoflavone on testosterone level, total Leydig cells, and total spermatogenic cells in the seminiferous tubules of male rats. Diet was given as isonitrogen and isocaloric with 10% of dietary protein from casein. Twenty five male of Sprague Dawley weaning rats (21 days old) were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different levels (dosages). The treatment was conducted for 2 months. The treatment of isoflavone-riched soybean flour with higher dosage of isoflavone increased the testosterone levels in the serum and the total Leydig cells in the seminiferous tubules of male rats. The optimum dosage of isoflavone was 1.5 mg/day resulted in the highest total spermatogenic cells of rat testes. The treatment isoflavone-riched soybean flour with 1.5 mg isoflavone/day on male rats resulted in the testosteron level of 2.96±0.45 ng/ml, total Leydig cells 70.22±9.34; while the number of spermatogonia, spermatocytes, early spermatids, late spermatids, and total spermatogenic cells were 48.44±4.82, 60.00±3.43, 221.56± 16.12, 164.33±17.94, and 494.33±32.94, respectively.
Co-Authors Admi Syarif Agus Wantoro Elvira Rossalia Kambu Fajrin Nuraida Hendri Busman Kamiliah Tsany Khairun Nisa Kurnia Muludi M. Kanedi Nuning Nurcahyan Nuning Nurcahyani Ratna Dewi Puspita Rismayani Saridewi Siti Umairoh Sri Ratna Sulistiyanti Sumayyah Annida Sussi Astuti